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ncode vilo mirna cdna synthesis kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher ncode vilo mirna cdna synthesis kit
    Ncode Vilo Mirna Cdna Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ncode+vilo+mirna+cdna+synthesis+kit/pm39529187-89-12-18
    Average 90 stars, based on 1 article reviews
    ncode vilo mirna cdna synthesis kit - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    cDNA Synthesis:

    Article Title: Noncoding RNAs: Possible Players in the Development of Fluorosis
    Article Snippet: .. 500 ng of miRNA was converted to cDNA using NCode VILO miRNA cDNA Synthesis Kit (Invitrogen, USA). .. Relative quantification of miRNA was performed on Applied Biosystems 7300 real time PCR system using EXPRESS SYBR GreenER qPCR SuperMix containing universal reverse primers (Invitrogen, USA) and forward customized primers of miR-124/miR-155 ( ).

    Article Title: MSC–extracellular vesicle microRNAs target host cell-entry receptors in COVID-19: in silico modeling for in vivo validation
    Article Snippet: .. Following the manufacturer’s instructions, cDNA was synthesized from miRNAs using an Invitrogen NCode VILO miRNA cDNA Synthesis Kit and a Bio-Rad T100 Thermal Cycler (USA) with U6 as an internal control for miRNA quantification [ ]. ..

    Article Title: Suppression of Expression of Heat Shock Protein 70 by Gefitinib and Its Contribution to Pulmonary Fibrosis
    Article Snippet: Antibodies against HSP27, HSP47, HSP60 and HSP90 were from Stressgene (San Francisco, CA). .. The NCode VILO miRNA cDNA synthesis kit and Lipofectamine (TM2000) were obtained from Invitrogen (Carlsbad, CA). .. A771726, gefitinib, imatinib, amiodarone, L-hydroxyproline, azophloxin and aniline blue were from WAKO Pure Chemicals (Tokyo, Japan).

    Article Title: MSC-extracellular vesicle microRNAs target host cell-entry receptors in COVID-19: in silico modeling for in vivo validation.
    Article Snippet: .. Following the manufacturer’s instructions, cDNA was synthesized from miRNAs using an Invitrogen NCode VILO miRNA cDNA Synthesis Kit and a Bio-Rad T100 Thermal Cycler (USA) with U6 as an internal control for miRNA quantification [33]. ..

    Article Title: Sex-Biased miRNAs in Gonad and Their Potential Roles for Testis Development in Yellow Catfish
    Article Snippet: Quantitative real-time PCR (qRT-PCR) with iTaq Universal SYBR Green Supermix (Bio-Rad) was performed to profile the expression level of arbitrarily selected miRNAs in three libraries, as previously described . .. Briefly, 1 μg of total RNA was reverse transcribed by using NCode VILO miRNA cDNA Synthesis Kit (Invitrogen). .. All real-time reactions were run using the CFX96 Touch Real-Time PCR Detection System (Bio-Rad).

    Article Title: The MicroRNA and MessengerRNA Profile of the RNA-Induced Silencing Complex in Human Primary Astrocyte and Astrocytoma Cells
    Article Snippet: .. Poly-A tailing of the miRNA in the total RNA sample and cDNA synthesis was performed using the NCode VILO miRNA cDNA synthesis kit (Invitrogen, Burlington, ON, Canada) according to the manufacturer's instructions. ..

    Article Title: A Decrease of Brain MicroRNA-122 Level Is an Early Marker of Cerebrovascular Disease in the Stroke-Prone Spontaneously Hypertensive Rat
    Article Snippet: Then, RT-PCR of miR-122 was performed through the use of a specific TaqMan microRNA assay (Applied Biosystems, Foster City, USA) using miRU87 as control. .. For this purpose, 1 μ g of purified RNA was mixed with 3 μ l of NCode VILO miRNA cDNA Synthesis Kit (Life Technologies) and 1x RT-primer (Life Technologies) in a total reaction volume of 20 μ l. Reaction was incubated at 16°C for 30 min., 42°C for 30 min, and 85°C for 5 min in a T-100 Thermal Cycler (Bio-Rad, Hercules, CA, USA). .. Then, 2 μ l of the RT reaction was added to 1 μ l of a specific TaqMan microRNA assay 20x and 10 μ l of TaqMan Universal PCR Master Mix II with UNG (Life Technologies) in a 20 μ l final volume.

    Article Title: MSCs-derived EVs protect against chemotherapy-induced ovarian toxicity: role of PI3K/AKT/mTOR axis.
    Article Snippet: .. The SensiFast cDNA synthesis kits (Sigma Bioline, UK) for RNA and the NCode VILO miRNA cDNA Synthesis Kit (Invitrogen, USA) for miRNA were used to synthesize complementary DNA (cDNA), following the manufacturer’s protocol. .. Next, quantitative PCR (qPCR) was performed using Maxima SYBR Green/ROX qPCR master mix (2x) (Thermo Scientific, USA).

    Synthesized:

    Article Title: MSC–extracellular vesicle microRNAs target host cell-entry receptors in COVID-19: in silico modeling for in vivo validation
    Article Snippet: .. Following the manufacturer’s instructions, cDNA was synthesized from miRNAs using an Invitrogen NCode VILO miRNA cDNA Synthesis Kit and a Bio-Rad T100 Thermal Cycler (USA) with U6 as an internal control for miRNA quantification [ ]. ..

    Article Title: MSC-extracellular vesicle microRNAs target host cell-entry receptors in COVID-19: in silico modeling for in vivo validation.
    Article Snippet: .. Following the manufacturer’s instructions, cDNA was synthesized from miRNAs using an Invitrogen NCode VILO miRNA cDNA Synthesis Kit and a Bio-Rad T100 Thermal Cycler (USA) with U6 as an internal control for miRNA quantification [33]. ..

    Control:

    Article Title: MSC–extracellular vesicle microRNAs target host cell-entry receptors in COVID-19: in silico modeling for in vivo validation
    Article Snippet: .. Following the manufacturer’s instructions, cDNA was synthesized from miRNAs using an Invitrogen NCode VILO miRNA cDNA Synthesis Kit and a Bio-Rad T100 Thermal Cycler (USA) with U6 as an internal control for miRNA quantification [ ]. ..

    Article Title: MSC-extracellular vesicle microRNAs target host cell-entry receptors in COVID-19: in silico modeling for in vivo validation.
    Article Snippet: .. Following the manufacturer’s instructions, cDNA was synthesized from miRNAs using an Invitrogen NCode VILO miRNA cDNA Synthesis Kit and a Bio-Rad T100 Thermal Cycler (USA) with U6 as an internal control for miRNA quantification [33]. ..

    Reverse Transcription:

    Article Title: Sex-Biased miRNAs in Gonad and Their Potential Roles for Testis Development in Yellow Catfish
    Article Snippet: Quantitative real-time PCR (qRT-PCR) with iTaq Universal SYBR Green Supermix (Bio-Rad) was performed to profile the expression level of arbitrarily selected miRNAs in three libraries, as previously described . .. Briefly, 1 μg of total RNA was reverse transcribed by using NCode VILO miRNA cDNA Synthesis Kit (Invitrogen). .. All real-time reactions were run using the CFX96 Touch Real-Time PCR Detection System (Bio-Rad).

    Purification:

    Article Title: A Decrease of Brain MicroRNA-122 Level Is an Early Marker of Cerebrovascular Disease in the Stroke-Prone Spontaneously Hypertensive Rat
    Article Snippet: Then, RT-PCR of miR-122 was performed through the use of a specific TaqMan microRNA assay (Applied Biosystems, Foster City, USA) using miRU87 as control. .. For this purpose, 1 μ g of purified RNA was mixed with 3 μ l of NCode VILO miRNA cDNA Synthesis Kit (Life Technologies) and 1x RT-primer (Life Technologies) in a total reaction volume of 20 μ l. Reaction was incubated at 16°C for 30 min., 42°C for 30 min, and 85°C for 5 min in a T-100 Thermal Cycler (Bio-Rad, Hercules, CA, USA). .. Then, 2 μ l of the RT reaction was added to 1 μ l of a specific TaqMan microRNA assay 20x and 10 μ l of TaqMan Universal PCR Master Mix II with UNG (Life Technologies) in a 20 μ l final volume.

    Incubation:

    Article Title: A Decrease of Brain MicroRNA-122 Level Is an Early Marker of Cerebrovascular Disease in the Stroke-Prone Spontaneously Hypertensive Rat
    Article Snippet: Then, RT-PCR of miR-122 was performed through the use of a specific TaqMan microRNA assay (Applied Biosystems, Foster City, USA) using miRU87 as control. .. For this purpose, 1 μ g of purified RNA was mixed with 3 μ l of NCode VILO miRNA cDNA Synthesis Kit (Life Technologies) and 1x RT-primer (Life Technologies) in a total reaction volume of 20 μ l. Reaction was incubated at 16°C for 30 min., 42°C for 30 min, and 85°C for 5 min in a T-100 Thermal Cycler (Bio-Rad, Hercules, CA, USA). .. Then, 2 μ l of the RT reaction was added to 1 μ l of a specific TaqMan microRNA assay 20x and 10 μ l of TaqMan Universal PCR Master Mix II with UNG (Life Technologies) in a 20 μ l final volume.



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